Antioxidant and Free Radical Scavenging Activity of Methanolic Extract of (Hordeum vulgare) Barley

Abstract

Aim: The aim of the study is to investigate the antioxidant activity of (HORDEUM VULGARE) Barley. Materials and Methods Thebarley(Hordeumvulgare)leaves16-28cminheightwerewashedwith water, cutintopieces, and dried by a hot air dryer at 70ºC for 24 hour and methanolic extract was prepared. Sample were dissolved in parts of 80% methanol in 1:3 ratios and placed in hot water bath shaker for 16 to 18 hours followed by filtration using muslin cloth. The filtrate was stored and the residue was re-extracted by same procedure. Reaction mixture was added to the methanolic extract and incubated at room temperature for 5minutes.Theabsorbance of the mixture at 560 nm was measured with aspectrophotometer. Results Methanol is likewise an excellent solvent, because of its high polarity. Methanol was observed simpler to penetrate the cell membrane to extract the intracellular elements from the plant material. In PMS-NADH-NBT system, superoxide anion derived from dissolved oxygen by way of PMS-NADH coupling response reduces NBT. Methanolic extract of barley in concentration of 200μg/mL shows 85% inhibition of superoxide radical. Whereas the methanolic extract
in concentration of 30–120 μg/mL inhibit hydroxyl free radical generation in concentration dependentmanner. Conclusion The present study revealed that barley extracts has potent antioxidant activity, achieved by free radical scavenging and reducing power assays. Methanolic extracts of extracts were investigated for anti-free radical activity by superoxide and hydroxyl assay.z