WESTERN BLOT ASSAY OF SELECTED PATIENTS BLOOD INFECED WITH HIV : IN AND AROUND SALEM DISTRICT, TAMILNADU, INDIA.

Abstract

The human immunodeficiency virus (HIV) is the etiological agent of acquired immunodeficiency syndrome and demonstraction of an antibody response specifically directed against HIV proteins is accepted as evidence of infection. Westernblot is the most commonly used test to conform Ahiv-I infection to perform the westerNblot the HIV-I antigens are separated on the basis of their molecular weights and antibodies to each components aare detected as distint bands. The following HIV-I antigens are currently identified in the westenblot ENVELOP( gp160, gp120,gp41)GROUP ANTIGENS (p55,p40,p24,p18)and POLYMERASE(p51,p65 andp31). To
determine the indeterminate HIV-I and HIV-2 western blot results of HIV infected individuals. The westernblot is the confirmatory test for HIV infection. The infected blood samples had tested should have atleast one HIV characteristic band and any one oh HIV-2 band. We obtained information regarding HIV risk factor and peripheral blood specimen for following test , HIV-TRI-DOT for detection of antibodies in the blood samples.