The Molecular Profiling and HCV RNA Quantification to Study the Distribution of Different HCV Genotypes in Accordance to Geographical Condition

Abstract

The HCV RNA quantification provides insight for treating and curing the HCV (Hepatitis C Virus) and HCC (Hepatocellular Carcinoma). This study deals with the molecular profiling of HCV RNA viral load and the molecular investigation of the HCV RNA genotypes. The distribution of various genotypes among the population is studied in this paper. Total 94 EDTA blood samples were taken from suspected HCV reactive patients from the different hospitals including OPDs and IPDs of in Dehradun, Uttarakhand, India. The RNA extraction was performed by the QIAamp Viral RNA mini kit (cat. no. 52904) and the HCV RNA quantification was performed by Rotor Gene Q 5 Plex Real Time PCR machine. The HCV genotypic characterization was performed by the Real Time PCR Technology utilizing Sansure (Korea) Biotech kit. Out of 94 samples collected for HCV RNA quantification, 44.7% (42 cases) were with target was not detected and 55.3%, i.e., 52 cases were with high viral load. HCV genotype 3 was found to be the most prevalent in 65.62% of the cases i.e. 21 cases in total. The presence of HCV genotype varies within the different geographical conditions because of the differences in the major causative factors. The HCV RNA viral load was quantified in 52 samples with high HCV RNA viral load and their titer values were observed in between the 34 IU/ml to 1.10×108 IU/ml and the other 42 samples were not considered for genotyping because of low viral load in them.